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PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER OF MANGIFERA INDICA (OPIORO MANGO)

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TABLE OF CONTENT
CONTENT                                                                             
Title page                                                                              
Declaration                                                                                               
Certification                                                                                      
Dedication                                                                                                
Acknowledgment                                                                                    
Table of content                                                                             
Abstract                                                                                           

CHAPTER ONE
1.0    INTRODUCTION                                                                            
OBJECTIVE OF THIS RESEARCH                                    
1.1    LITERATURE REVIEW                                                        
1.2    GENERAL USES AND IMPORTANCE OF
MANGIFERA INDICA                                                          
1.3    MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS 
FLOWERS                                                                                      
CHAPTER TWO
2.0    MATERIALS AND METHODS                                            
2.1    MATERIALS                                                                          
2.2    EQUIPMENTS AND APPARATUS                                              
2.3    CHEMICAL REAGENTS                                                      
2.4    PHYTOCHEMICAL SCREENING (QUANTITATIVE        
ANALYSIS)                                                                            
2.5    DETERMINATION OF SAPONIN                                       
2.6    DETERMINATION OF ALKALOID                                              
2.7    DETERMINATION OF TANINS                                          
2.8    DETERMINATION OF MOISTURE CONTENT                
2.9    DETERMINATION OF TOTAL ASH                                   
2.10  CARDIAC GLYCOSIDE                                                       
2.11  TEST FOR ANTHRACENE GLYCOSIDES                       
2.12  TERPENOIDS                                                                       
2.13  pH AT 200C (10%)                                                                
CHAPTER THREE
TABLE 3.1 ORGANOLEPTIC  TEST  RESULT                          
TABLE 3.2 RESULT OF THE  PHYTOCHEMICAL  ANALYSIS 
OF FLOWER AND STALK OF Mangifera Indica     
TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO 
GRAM POSITIVE BACTERIA IN TWO CRUDE 
EXTRACTS OF FLOWER AND STALK OF 
Mangifera indica                                                          
TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF 
TWO GRAM NEGATIVE BACTERIA IN TWO 
CRUDE EXTRACTS OF FLOWER AND STALK 
OF Mangifera indica                                                    
TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF
TWO FUNGI IN TWO CRUDE EXTRACTS 
OF FLOWER AND STALK 
OF Mangifera indica                                                    
TABLE 3.6 RESULT OF MINIMUM INHIBITORY 
CONCENTRATION 
(MIC) OF CRUDE EXTRACT OF FLOWER 
AND STALK 
OF Mangifera indica                                                    
CHAPTER FOUR
DISCUSSION                                                              
CONCLUSION                                                             
RECOMMENDATION                                                 
REFERENCES                                                            
APPENDIX                                                                           

ABSTRACT
Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%,  Alkaloid      7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid  6.11% +0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%,  pH 3.84% + 0.0529%. Cardiac Glycoside  7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureusS. pyogensEscherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited. 


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