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HYPOLIPIDEMIC EFFECT OF TIGERNUT (Cyperus esculentus) ON ALBINO RATS

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ABSTRACT

The effect of intake of tigernut extract was studied. Doses of 0.2ml and 0.8ml of tigernut (cyperus esculentus) extract as well as the administration of 0.3ml questran (hypolipidemic drug) were orally administered to 6 weeks old male rats for 21 days. The effect of this administration on serum lipid levels such as cholesterol, triglyceride, HDL-cholesterol and LDL-cholesterol were studied. The result obtained indicate that tigernut causes significant (P<0.05) decrease in the cholesterol, triglyceride and LDL-cholesterol and a significant (p<0.05) increase in the HDL and so exhibit hypolipidemic properties.
 

TABLE OF CONTENTS

Title page                                    i
Certification page                            ii
Dedication                                    iii
Acknowledgement                            iv
Abstract                                    v
Table of contents                            vi
List of figures                                x

CHAPTER ONE:
1.0    Introduction                            1

1.1    Background of the study                    1
1.2    Statement of problem                    4
1.3    Objective of the study                    4
1.4    Hypothesis or research questions            5
1.5    Significance of the study                    5

CHAPTER TWO: 
2.0    Literature Review                            6

2.1    History of Tigernut                            6
2.2    Uses of Tigernut                             7
2.3    Health benefits of Tigernuts                    7
2.4    Nutritional value of Tigernut                    11
2.5    Lipids and its effects                            12
2.6    Effect of Tigernut on metabolism                14

CHAPTER THREE
3.0    Materials and method                            16

3.1    Material                                    16
3.2    Chemical Reagents                            16
3.3    Methods                                    17
3.3.1        Collection of sample                        17
3.3.2        Extraction of Tigernut oil                    17
3.3.3        Care and Management of experimental animal    18
3.3.4        Experimental design                        18
3.3.5        Collection of blood samples for Biochemical   analysis                        20
3.3.6        Determination of cholesterol                20
3.3.7        Determination of triglyceride                22
3.3.8        Determination of HDL cholesterol            24
3.3.9        Determination of LDL cholesterol             26

CHAPTER FOUR:
4.0    Results                                    27

CHAPTER FIVE
5.0    Discussion, Conclusion and Recommendation        33
5.1    Discussion                                    33
5.1.1    Effect of tigernut extract on serum cholesterol of rats                        33
5.1.2    Effect of Tigernut extract on serum triglyceride of rats                        34
5.1.3    Effect of Tigernut extract on serum HDL of rats    34
5.1.4    Effects of Tigernut on serum LDL of rats        34
5.1.5    Effect of Tigernut extract on body weight of rats    35
5.2    Conclusion                                    35
5.3    Recommendation                            36
References                                          7
Appendix                                          40 

LIST OF FIGURES

figure 1:        Serum cholesterol concentration     28
figure II:     Serum triglyceride concentration 29
figure III:    Serum HDL-C concentration         30
figure iv:            Serum LDL-C concentration         31
figure v:            Percentage increase in body weight    32

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