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EVALUATION OF CURRENT TECHNIQUES IN DIAGNOSES OF HUMAN IMMUNODEFICIENCY VIRUS (HIV)

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ABSTRACT

    A study was carried to ascertain the potency of some techniques used in diagnosis of Human Immunodeficiency virus (HIV).  Two hundred and five samples (203) were collected from the patients from the university of Nigerian teaching hospital and was analysed using Elisa kit, which has the ability to detect antibodies and antigens in the patients serum, the Western blots were used as the confirmatory test, to the Elisa test, In case where there is false positive of Elisa test, it is specific and sensitive, Radioimmunoasay looks for the antigens which are radioactively label.  The patients result shows that 154 patients out of the 205 diagnosed were negative and only 51 patients were positive.  The Elisa kit were best used because of  its sensitivity and specificity.  It is very cheap and can be easily seen in the market, unlike the Radiommunoassay which are rare and very expensive to be used.

LIST OF TABLE

Table 1:    Number of both sexes, age and the results of patience diagnosed.
Table 2:    Number of both positive and negative Results
Table 3:    Numbers of negative results with their percentage
Table 4:    Number of negative results with their percentage
Table 5:    Rate of infection among the study group

TABLE OF CONTENT

Title page                                    
Certification                                    
Acknowledgment                                 
Dedication                                    
Abstract                                    
List of tables        
                        
CHAPTER ONE                        
1.0    Introduction                                
1.1    Hypothesis                                
1.2    Aims and Objectives                        
1.3    Statement of problem                        
1.4    Limitation of study
                        
CHAPTER TWO
2.0    Literature review                            
2.1    Pathogenesis of HIV infection                    
2.2    Epidemiology of HIV                        
2.3    Transmission of HIV infection                    
2.4    Viral replication of HIV                        
2.5    Symptoms of HIV infection                    
2.6    Life cycle of HIV                            
2.7    Facts about HIV/AIDS
                        
CHAPTER THREE                
3.0    Materials and Method                        
3.1    Current  diagnosis of HIV infection                
3.2    Laboratory diagnosis                        
3.3    Radiomminoassay                            
3.4    Enzymes Linked immunosorbentassay Elisa            
3.5    Western blot    
                            
CHAPTER FOUR
4.0    Results    

CHAPTER FIVE                            
5.0      Discussion and conclusion
5.1.     Conclusion
5.2    Recommendation                    
    References                                
    Appendices    

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