ABSTRACT
The effect of intake of tigernut extract was studied. Doses of 0.2ml and 0.8ml of tigernut (cyperus esculentus) extract as well as the administration of 0.3ml questran (hypolipidemic drug) were orally administered to 6 weeks old male rats for 21 days. The effect of this administration on serum lipid levels such as cholesterol, triglyceride, HDL-cholesterol and LDL-cholesterol were studied. The result obtained indicate that tigernut causes significant (P<0.05) decrease in the cholesterol, triglyceride and LDL-cholesterol and a significant (p<0.05) increase in the HDL and so exhibit hypolipidemic properties.TABLE OF CONTENTS
Title page iCertification page ii
Dedication iii
Acknowledgement iv
Abstract v
Table of contents vi
List of figures x
CHAPTER ONE:
1.0 Introduction 1
1.1 Background of the study 1
1.2 Statement of problem 4
1.3 Objective of the study 4
1.4 Hypothesis or research questions 5
1.5 Significance of the study 5
CHAPTER TWO:
2.0 Literature Review 6
2.1 History of Tigernut 6
2.2 Uses of Tigernut 7
2.3 Health benefits of Tigernuts 7
2.4 Nutritional value of Tigernut 11
2.5 Lipids and its effects 12
2.6 Effect of Tigernut on metabolism 14
CHAPTER THREE
3.0 Materials and method 16
3.1 Material 16
3.2 Chemical Reagents 16
3.3 Methods 17
3.3.1 Collection of sample 17
3.3.2 Extraction of Tigernut oil 17
3.3.3 Care and Management of experimental animal 18
3.3.4 Experimental design 18
3.3.5 Collection of blood samples for Biochemical analysis 20
3.3.6 Determination of cholesterol 20
3.3.7 Determination of triglyceride 22
3.3.8 Determination of HDL cholesterol 24
3.3.9 Determination of LDL cholesterol 26
CHAPTER FOUR:
4.0 Results 27
CHAPTER FIVE
5.0 Discussion, Conclusion and Recommendation 33
5.1 Discussion 33
5.1.1 Effect of tigernut extract on serum cholesterol of rats 33
5.1.2 Effect of Tigernut extract on serum triglyceride of rats 34
5.1.3 Effect of Tigernut extract on serum HDL of rats 34
5.1.4 Effects of Tigernut on serum LDL of rats 34
5.1.5 Effect of Tigernut extract on body weight of rats 35
5.2 Conclusion 35
5.3 Recommendation 36
References 7
Appendix 40
LIST OF FIGURES
figure 1: Serum cholesterol concentration 28
figure II: Serum triglyceride concentration 29
figure III: Serum HDL-C concentration 30
figure iv: Serum LDL-C concentration 31
figure v: Percentage increase in body weight 32
