The project is titled “The Role of Advertisement in Newspaper Patronage: A case study of Nigerian Tribune Newspaper; a stable of African Newspapers of Nigeria (ANN) PLC Ibadan. The purpose of this research work is to find out the role of Advertisement in Newspaper patronage with a special focus on Tribune Newspaper. Survey method was adopted as research methodology with questionnaire as data collection instrument; Inhabitants of two local governments were randomly selected in Osun state. Boluwaduro and Boripe Local Governments respectively, 60 respondents were chosen as sample size. The review of existing literature revealed that no organization can survive in a competition driven without advertisement. The history and revolution of information technology have forced a lot of media organizations to rethink the way of attracting patronage and maintain existing ones. The analysis of data gathered shows that majority of the respondents believed that newspaper advertisement has helped in increasing the sales of newspaper especially for Nigeria Tribune Newspaper. The researcher concluded that advertisement by newspaper house is a sine-qua-non to purchase of Newspapers in Nigeria. It is recommended that NUJ and NPAN should ensure that media houses should uphold high standard of professional practice and adhere strictly to the ethics of the profession in lieu of the huge amount of money realized from Advertisers in the Newspapers.